Coding

Part:BBa_K5241007:Design

Designed by: yingmei tang   Group: iGEM24_ECIB-PKU   (2024-09-25)


PelB-PkhppD-6xH-20b（+）


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 43
    Illegal EcoRI site found at 52
    Illegal EcoRI site found at 967
    Illegal PstI site found at 925
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 43
    Illegal EcoRI site found at 52
    Illegal EcoRI site found at 967
    Illegal PstI site found at 925
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 43
    Illegal EcoRI site found at 52
    Illegal EcoRI site found at 967
    Illegal XhoI site found at 1075
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 43
    Illegal EcoRI site found at 52
    Illegal EcoRI site found at 967
    Illegal PstI site found at 925
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 43
    Illegal EcoRI site found at 52
    Illegal EcoRI site found at 967
    Illegal PstI site found at 925
    Illegal NgoMIV site found at 342
    Illegal NgoMIV site found at 1140
    Illegal AgeI site found at 319
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 316


Design Notes

none


Source

Pseudomonas_koreensis

References